Journal: PLoS ONE
Article Title: Construction of a BALB/c-Nu Mouse Model of Invasive Bladder Carcinoma and Preliminary Studies on the Treatment of Bladder Tumors through Internal Iliac Arterial Infusion of Albumin-Bound Arsenic Trioxide (As 2 O 3 )
doi: 10.1371/journal.pone.0124959
Figure Lengend Snippet: (a), (b) Gross photographs show the mouse bladder was invaded by the tumor cells and enlarged. And bladder wall was diffuse thickening. (c) Pathological examination showed tumor with extensive serous membrane, muscularis propria diffuse invasion, tumor cells with loss of polarity and arranged disorder. This showed significant cytologic atypia whithout obvious necrosis. And it was solid tumor on the left side (H×200). (d) Tumor cells invading the submucosa and muscular layer on the left side. The enlarged nuclei of tumor cells showed pleomorphism with hyperchromatic, irregular nuclei, inconsistency of shape and size, irregular clumped chromatin with uneven distribution, increased ratio of nucleus to cytoplasm, and prominent enlarged nucleoli. And it was normal mucosa on the right side (H×400).
Article Snippet: The human bladder cancer cell line EJ, obtained from Nanjing KeyGen Biotech Co., was cultured in a RPMI 1640 medium with 10% fetal bovine serum (Sigma Chemicals).
Techniques: Membrane